Related Experiment Video
Updated: Feb 7, 2026
08:45
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
14.2K
Statin resistance in Candida glabrata
Mishal Subhan1,2, Rani Faryal2, Ian Macreadie3
1School of Science, RMIT University, Bundoora, VIC, 3083, Australia.
Biotechnology Letters
|July 29, 2018
Summary
This study investigated statin resistance mechanisms using Candida glabrata. Findings suggest HMGCR overexpression or non-HMGCR mechanisms contribute to statin resistance, offering insights into reduced drug efficacy.
Area of Science:
- Biochemistry
- Molecular Biology
- Microbiology
Background:
- Reduced efficacy of statins is observed in some individuals.
- The underlying mechanisms of statin resistance are not fully understood.
- No statin-resistance mutations in the catalytic domain of HMGCR have been previously reported.
Purpose of the Study:
- To identify statin-resistance mutations.
- To investigate the mechanisms of statin resistance.
- To utilize Candida glabrata as a model organism for studying statin resistance.
Main Methods:
- Culturing Candida glabrata in media containing lovastatin, simvastatin, or atorvastatin to generate resistant mutants.
- Purifying individual resistant mutants for detailed analysis.
- Performing gene sequencing on HMGCR in resistant mutants.
Main Results:
- Gene sequencing revealed no mutations in the catalytic domain of HMGCR in any of the resistant mutants.
- Two resistant isolates showed overexpression of HMGCR, indicating increased enzyme production as a resistance mechanism.
- One mutant exhibited unaltered HMGCR activity, suggesting alternative resistance pathways like increased drug efflux.
Conclusions:
- Candida glabrata serves as an effective model organism for studying statin resistance.
- Further research is needed to elucidate the precise molecular mechanisms driving statin resistance.
More Related Videos
Related Concept Videos
01:20Lipid-Lowering Drugs: Statins and Miscellaneous Agents
1.4K
Hyperlipidemia, a medical condition often referred to as high cholesterol, is characterized by abnormally elevated levels of lipids in the bloodstream. When present in excess, these lipids, specifically cholesterol and triglycerides, can lead to serious health complications, often involving cardiovascular diseases. Illnesses like atherosclerosis, heart attacks, and pancreatitis have all been linked to untreated hyperlipidemia. This means controlling and regulating cholesterol and triglyceride...
1.4K
01:22Resistivity
4.6K
When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.6K
01:19Resistance
6.0K
When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
6.0K
01:16Equivalent Resistance
987
In circuit analysis, situations often arise where resistors are neither in series nor parallel configurations. To tackle such scenarios, three-terminal equivalent networks like the wye (Y) (Figure 1 (a)) or tee (T) and delta (Δ) (Figure 1 (b)) or pi (π) networks come into play. These networks offer versatile solutions and are frequently encountered in various applications, including three-phase electrical systems, electrical filters, and matching networks.
987
01:25Resistance and Conductance
518
A conductor's DC resistance at a given temperature is influenced by its resistivity, length, and cross-sectional area. Resistivity is an inherent property of the conductor material, with annealed copper serving as the international standard for measurement. For instance, the resistivity of hard-drawn aluminum at 20 degrees Celsius is 61% of the standard conductivity of annealed copper.
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
518
01:21Rolling Resistance
669
When a solid cylinder rolls steadily on a rigid surface, the normal force applied by the surface on the cylinder is perpendicular to the tangent at the contact point. However, since no materials are entirely rigid, the surface's reaction to the cylinder involves a range of normal pressures.
For instance, imagine a hard cylinder rolling on a comparatively soft surface. The cylinder's weight compresses the surface beneath it. As the cylinder moves, the material in front of it slows down due to...
For instance, imagine a hard cylinder rolling on a comparatively soft surface. The cylinder's weight compresses the surface beneath it. As the cylinder moves, the material in front of it slows down due to...
669