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Methods to Study the Role of Cdc42, Rac1, and Rac2 in B-Cell Cytoskeletal Responses
Natalija Gerasimcik1, Lisa S Westerberg2, Eva Severinson3
1Department of Dental Medicine, Division of Periodontology, Karolinska Institutet, Huddinge, Sweden.
Abstract:
B-cell migration and adhesion are critical to form a germinal center response, the site for B-cell production of high-affinity antibodies. Here, we describe two assays that can be used to examine B-cell cytoskeletal responses needed during the germinal center response: B-cell spreading and homotypic adhesion. Spreading of B cells is dependent on Cdc42, while Rac1 and Rac2 are necessary for homotypic adhesion. These in vitro assays can be used to examine functional responses of B cells mediated by the cell cytoskeleton, for example when comparing B cells from different gene knockout animals.
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