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Updated: Feb 7, 2026

Derivation of Human Embryonic Stem Cells by Immunosurgery
Published on: December 13, 2007
A Practical Protocol for the Conditional Depletion of Rho Isoforms in Human Embryonic Stem Cells
1Institute for Frontier Life and Medical Science, Kyoto University, Kyoto, Japan. mohgushi@infront.kyoto-u.ac.jp.
Abstract:
Human pluripotent stem cells indefinitely proliferate and survive in culture while retaining genomic integrity, providing a unique opportunity to study human molecular biology. Here, we introduced an RNA interference-based protocol of inducible gene silencing in human embryonic stem cells, which has several advantages in handling simplicity/convenience, cost/time performance, and applicability. Using this method, we had succeeded to elucidate the isoform-unique roles of Rho-family small GTPases in human embryonic stem cells.
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