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Updated: Feb 7, 2026

Author Spotlight: Non-Invasive High-Resolution Measurement of Chlorophyll Synthesis During De-Etiolation
Published on: January 12, 2024
Simulation of chlorophyll fluorescence rise and decay kinetics, and P700-related absorbance changes by using a
T K Antal1, A Maslakov2, O V Yakovleva2
1Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russian Federation, 119991. taras@biophys.msu.ru.
Abstract:
A model of primary photosynthetic reactions in the thylakoid membrane was developed and its validity was tested by simulating three types of experimental kinetic curves: (1) the light-induced chlorophyll a fluorescence rise (OJIP transients) reflecting the stepwise transition of the photosynthetic electron transport chain from the oxidized to the fully reduced state; (2) the dark relaxation of the flash-induced fluorescence yield attributed to the QA- oxidation kinetics in PSII; and (3) the light-induced absorbance changes near 820 or 705 nm assigned to the redox transitions of P700 in PSI. A model was implemented by using a rule-based kinetic Monte-Carlo method and verified by simulating experimental curves under different treatments including photosynthetic inhibitors, heat stress, anaerobic conditions, and very high light intensity.
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