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Isoprostanoids quantitative profiling of marine red and brown macroalgae
Claire Vigor1, Guillaume Reversat1, Amandine Rocher1
1Institut des Biomolécules Max Mousseron IBMM - UMR 5247 - CNRS - UM - ENSCM, Faculté de pharmacie 15, Avenue Charles Flahault, 34060 Montpellier CEDEX 2, France.
Food Chemistry
|August 2, 2018
Summary
Seaweed farming is expanding, but algae face oxidative stress. This study identifies key oxidative stress biomarkers in macroalgae, finding their levels increase under copper exposure.
Area of Science:
- Marine Biology
- Environmental Chemistry
- Biochemistry
Background:
- Seaweed aquaculture is growing globally for human and animal consumption.
- Macroalgae inhabit environments with frequent biotic and abiotic changes, leading to oxidative stress (OS).
- Isoprostanoid derivatives are significant biomarkers for OS.
Purpose of the Study:
- To profile isoprostanoids in red and brown macroalgae.
- To investigate the impact of copper exposure on isoprostanoid levels in macroalgae.
Main Methods:
- Quantification of seven phytoprostanes, three phytofuranes, and four isoprostanes.
- Utilized a novel micro-liquid chromatography-tandem mass spectrometry (micro-LC-MS/MS) method.
- Exposed macroalgae to heavy metal (copper) stress for 24 hours.
Main Results:
- Isoprostanoid content varied significantly across different macroalgae species.
- Ent-16(RS)-9-epi-ST-Δ14-10-PhytoF and the sum of 5-F2t-IsoP and 5-epi-5F2t-IsoP were major compounds.
- Copper exposure generally increased isoprostanoid concentrations, confirming OS induction.
- An exception was observed with a decrease in ent-9-L1-PhytoP in L. digitata under stress.
Conclusions:
- Established a method for quantifying OS biomarkers in macroalgae.
- Demonstrated that copper stress elevates isoprostanoid levels in most studied macroalgae.
- Highlighted the potential of isoprostanoids as indicators of environmental stress in marine ecosystems.
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