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Updated: Feb 7, 2026

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis
Published on: May 16, 2025
Spatial arrangement of proteins using scCro-tag: application for an in situ enzymatic microbead assay
Takaaki Kojima1, Jumpei Hata1, Hiroya Oka1
1a Graduate School of Bioagricultural Sciences , Nagoya University , Nagoya , Japan.
Abstract:
In natural systems, various metabolic reactions are often spatially organized to increase enzyme activity and specificity. Thus, by spatially arranging enzyme molecules in synthetic systems to imitate these natural systems, it is possible to promote a high rate of enzymatic turnover. In this present study, a normal and mutant form of the scCro DNA-binding protein were shown to bind orthogonally to specific recognition sequences under appropriate conditions. Furthermore, these DNA-binding tags were used to establish an enzyme assay system based on the spatial arrangement of transglutaminase and its substrate at the molecular level. Together, the results of the present study suggest that the scCro-tag may be a powerful tool to facilitate the synthetic spatial arrangement of proteins on a DNA ligand.
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