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The correlation of HCV RNA and HCV core antigen in different genotypes of HCV

Yu Xiang1, Xiao-Fei Lai1, Pu Chen1

  • 1Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Journal of Clinical Laboratory Analysis
|August 3, 2018
PubMed

Insights

Hepatitis C virus (HCV) core antigen (HCV cAg) shows a positive correlation with HCV RNA levels in genotype 1b infections. Chemiluminescent microparticle immunoassay (CMIA) detection of HCV cAg facilitates earlier diagnosis and treatment for HCV patients.

Area of Science:

  • Virology
  • Hepatology
  • Infectious Diseases

Background:

  • Hepatitis C virus (HCV) infection remains a significant global health concern.
  • Accurate and early diagnosis of HCV infection is crucial for effective treatment and management.
  • Understanding the correlation between HCV RNA and HCV core antigen (HCV cAg) across different genotypes is important for diagnostic strategies.

Purpose of the Study:

  • To investigate the correlation between HCV RNA and HCV cAg levels in patients with various HCV genotypes.
  • To compare the diagnostic performance of chemiluminescent microparticle immunoassay (CMIA) and enzyme-linked immunosorbent assay (ELISA) for HCV cAg detection.
  • To evaluate HCV cAg as a potential early diagnostic marker for HCV infection, particularly in genotype 1b.

Main Methods:

  • A cohort of 106 patients diagnosed with HCV infection was analyzed.
  • HCV genotypes were identified using PCR fluorescent probe technology.
  • HCV cAg expression was quantified using CMIA and qualitatively assessed via ELISA.

Main Results:

  • HCV genotype 1b was the predominant subtype (43.4%).
  • CMIA demonstrated a significantly higher positive rate (100%) for HCV cAg compared to ELISA (56%).
  • A positive correlation was observed between HCV RNA and HCV cAg in genotype 1b infections (r=0.894, P=0.04).

Conclusions:

  • HCV genotype 1b is the most prevalent subtype in the studied population.
  • CMIA offers superior sensitivity for HCV cAg detection, aiding in earlier diagnosis.
  • HCV cAg serves as a valuable early indicator for diagnosing HCV infection, especially in genotype 1b, correlating with viral load.
Abstract

Keywords:
HCV RNAHCV core antigencorrelationgenotypehepatitis C virus

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