Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Finishing Concrete01:18

Finishing Concrete

341
Concrete finishing starts immediately after the concrete has been placed and consolidated. The initial step, screeding, involves leveling the concrete surface by removing excess material to flush it with the formwork's top. Following this, bull float or darby are employed to smooth the surface further, effectively lower high spots, fill low areas, and ensure larger aggregate particles are embedded within the concrete. This preparation is critical before the appearance of bleed water, as its...
341
Formal Charges02:42

Formal Charges

40.6K
In some cases, there are seemingly more than one valid Lewis structures for molecules and polyatomic ions. The concept of formal charges can be used to help predict the most appropriate Lewis structure when more than one reasonable structure exists.
40.6K
Ions and Ionic Charges03:27

Ions and Ionic Charges

79.2K
In ordinary chemical reactions, the nucleus — which contains the protons and neutrons of each atom and thus identifies the element — remains unchanged. Electrons, however, can be added to atoms by transfer from other atoms, lost by transfer to other atoms, or shared with other atoms. The transfer and sharing of electrons among atoms govern the chemistry of the elements. During the formation of some compounds, atoms gain or lose electrons to form electrically charged particles called...
79.2K
DNA Isolation01:34

DNA Isolation

199.8K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
199.8K
Trends in Lattice Energy: Ion Size and Charge02:54

Trends in Lattice Energy: Ion Size and Charge

26.8K
An ionic compound is stable because of the electrostatic attraction between its positive and negative ions. The lattice energy of a compound is a measure of the strength of this attraction. The lattice energy (ΔHlattice) of an ionic compound is defined as the energy required to separate one mole of the solid into its component gaseous ions. For the ionic solid sodium chloride, the lattice energy is the enthalpy change of the process:
26.8K
Atomic Radii and Effective Nuclear Charge03:08

Atomic Radii and Effective Nuclear Charge

62.2K
The elements in groups of the periodic table exhibit similar chemical behavior. This similarity occurs because the members of a group have the same number and distribution of electrons in their valence shells.
62.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Symmetry-Enabled Optical Spin Initialization of Luminescent Organic Radical Doublet States.

Journal of the American Chemical Society·2026
Same author

How Symmetry Governs the Dihedral Angle Dependence of Intermolecular Spin-Orbit Coupling.

The journal of physical chemistry letters·2026
Same author

The Role of Chirality-Induced Spin Selectivity in Helicene-Based Photogenerated Radical Pairs.

Journal of the American Chemical Society·2026
Same author

Tracking Optical Phonon Dynamics in InP Nanocrystals via Transient Absorption and Femtosecond Stimulated Raman Spectroscopy.

ACS nano·2026
Same author

Identifying Electrolyte Reduction Intermediates in Lithium Metal Batteries with Spin Trapping.

Journal of the American Chemical Society·2026
Same author

Excited-State Symmetry Breaking Dynamics Driven by Mode-Resolved Jahn-Teller Distortion in Luminescent Tris(2,4,6-trichlorophenyl)methyl Radicals.

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: Feb 7, 2026

Real-time Tracking of DNA Fragment Separation by Smartphone
06:58

Real-time Tracking of DNA Fragment Separation by Smartphone

Published on: June 1, 2017

15.3K

Tracking Photoinduced Charge Separation in DNA: from Start to Finish.

Frederick D Lewis1, Ryan M Young1, Michael R Wasielewski1

  • 1Department of Chemistry and Institute for Sustainability and Energy at Northwestern , Northwestern University , Evanston , Illinois 60208-3113 , United States.

Accounts of Chemical Research
|August 3, 2018
PubMed
Summary
This summary is machine-generated.

Recent studies reveal key intermediates in DNA charge separation, showing hole transport is most efficient through adenine tracts. This advances understanding of photoinduced charge dynamics in DNA.

More Related Videos

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

790.5K
Harvesting Solar Energy by Means of Charge-Separating Nanocrystals and Their Solids
13:29

Harvesting Solar Energy by Means of Charge-Separating Nanocrystals and Their Solids

Published on: August 23, 2012

14.7K

Related Experiment Videos

Last Updated: Feb 7, 2026

Real-time Tracking of DNA Fragment Separation by Smartphone
06:58

Real-time Tracking of DNA Fragment Separation by Smartphone

Published on: June 1, 2017

15.3K
Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

790.5K
Harvesting Solar Energy by Means of Charge-Separating Nanocrystals and Their Solids
13:29

Harvesting Solar Energy by Means of Charge-Separating Nanocrystals and Their Solids

Published on: August 23, 2012

14.7K

Area of Science:

  • Biophysics
  • Photochemistry
  • Molecular Biology

Background:

  • Early studies on photoinduced charge separation in DNA suggested superexchange or tunneling mechanisms.
  • Subsequent research indicated hole transport over longer distances in DNA follows incoherent hopping.
  • Previous synthetic DNA constructs allowed measurement of charge transit times but not intermediate identification.

Purpose of the Study:

  • To identify key intermediates in DNA charge separation.
  • To elucidate the dynamics and efficiency of photoinduced charge separation from initiation to completion.
  • To investigate hole transport mechanisms in various DNA structures.

Main Methods:

  • Femtosecond transient absorption spectroscopy.
  • Stimulated Raman spectroscopy.
  • Utilizing synthetic DNA capped hairpin constructs with donor/acceptor chromophores.
  • Employing modified bases like 8-phenylethynylguanine (EG) for enhanced tracking.

Main Results:

  • Identified key intermediates in the charge separation process.
  • Established that initial charge injection depends on free energy and base stacking.
  • Demonstrated that hole transport in duplex DNA is most efficient through adenine tracts (A-tracts).
  • Detected guanine cation radicals and evidence for delocalized A-polarons in A-tracts.
  • Tracked hole transport across diverse DNA structures, including G-quadruplexes and locked nucleic acids.

Conclusions:

  • Conventional tunneling is not the primary mechanism for charge transport in DNA except at very short distances.
  • Hole transport dynamics in DNA are complex, involving hopping and delocalized intermediates like A-polarons.
  • Adenine tracts are crucial for efficient hole transport in DNA.
  • Advanced spectroscopic techniques enable detailed characterization of charge separation and transport in DNA.