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Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis.

Yaping Yang1, Yuheng Lin1, Jian Wang1

  • 1School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA, 30602, USA.

Nature Communications
|August 4, 2018
PubMed
Summary
This summary is machine-generated.

We developed a novel bifunctional dynamic control network using a sensor-regulator and RNA interference (RNAi) to simultaneously upregulate and downregulate cellular metabolism for engineered biosynthesis.

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Area of Science:

  • Synthetic biology
  • Metabolic engineering
  • Genetic circuit design

Background:

  • Developing genetic tools is crucial for engineering cellular functions and improving metabolic outputs.
  • Complex cellular environments require sophisticated control mechanisms for precise metabolic regulation.

Purpose of the Study:

  • To present a novel sensor-regulator and RNAi-based bifunctional dynamic control network.
  • To enable simultaneous upregulation and downregulation of cellular metabolism for engineered biosynthesis.

Main Methods:

  • Systematic verification and characterization of a promoter-regulator-mediated upregulation function.
  • Transduction of a downregulation function through RNA interference (RNAi).
  • Application of the dynamic control network to the phosphoenolpyruvate metabolic node in Escherichia coli.

Main Results:

  • Autonomous distribution of carbon flux between native metabolism and engineered muconic acid biosynthesis.
  • Achieved muconic acid biosynthesis yield of 1.8 g/L.
  • Demonstrated the utility of dynamic control for metabolic engineering.

Conclusions:

  • The developed bifunctional dynamic control network effectively regulates cellular metabolism for enhanced biosynthesis.
  • This approach allows for precise control over metabolic pathways, optimizing product yield.
  • The study provides insights into the applicability and conditions for dynamic control strategies in metabolic engineering.