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Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
07:49

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

The murine transforming growth factor-beta precursor

R Derynck, J A Jarrett, E Y Chen

    The Journal of Biological Chemistry
    |April 5, 1986
    PubMed

    Insights

    Researchers isolated mouse transforming growth factor-beta (TGF-beta) cDNAs, revealing the mature TGF-beta monomer and a homologous N-terminal region suggesting biological importance. This study details TGF-beta precursor structure and mRNA expression.

    Area of Science:

    • Molecular Biology
    • Cellular Biology
    • Biochemistry

    Background:

    • Transforming growth factor-beta (TGF-beta) is a polypeptide with dual roles, acting as both a mitogenic and growth inhibitory factor for various cell types.
    • Its function often depends on cooperation with other growth factors.

    Purpose of the Study:

    • To isolate and characterize cDNAs encoding the murine TGF-beta precursor.
    • To analyze the deduced amino acid sequence for conserved regions and potential functional domains.

    Main Methods:

    • Isolation of complementary DNAs (cDNAs) for the murine TGF-beta precursor.
    • Deduction of amino acid sequence from cDNA.
    • Sequence homology analysis comparing murine and human counterparts.
    • Northern hybridization to identify TGF-beta mRNA species.

    Main Results:

    • The 112-amino acid TGF-beta monomer is localized at the C-terminus of the precursor.
    • Two homologous regions were identified between murine and human precursors: one for the mature TGF-beta monomer and another at the N-terminus.
    • Northern hybridization revealed a major 2.5-kilobase TGF-beta mRNA and several minor species.

    Conclusions:

    • The N-terminal region of the TGF-beta precursor shows significant homology to the human counterpart, suggesting a crucial biological function.
    • The study provides insights into the structural organization and gene expression of TGF-beta.

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