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Updated: Feb 7, 2026

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
[Adventitious Buds Induction of Mentha haplocalyx Under Variation Inducted by NaN3]
Objective:
To establish the chemical mutagenesis in vitro system of Mentha haplocalyx,the effects of different plant hormone combination and Vc on the induction of mint stem adventitious buds and the mutation effects of different concentrations of NaN3 on their calli were studied.
Methods:
The internodes of mint used as the material,and based on the preliminary experiment, the effects of different concentrations of TDZ,6-BA,NAA and Vc on adventitious buds induction rate were researched. On the basis of screening the best induction formula, the Mentha haplocalyx calli were treated with different concentrations of NaN3( 0,2,4,6,8,10,12,14,16 mg/L).
Results:
The optimum medium for calli induction and adventitious buds formation was MS + 0. 1 mg / L TDZ + 0. 2 mg / L NAA + 1mg / L Vc + 30 g / L sucrose + 5. 5 g / L agar, the treatment concentration of NaN3 for LD50of calli induction was 14 mg / L for 10 d,or 12 mg / L for 20 d,or 10 mg / L for 30 d. Plantlet could differentiate from the calli treated with NaN3. By comparing to the regenerated plants,81 mutants had been selected.
Conclusion:
A chemical mutagenesis in vitro system for Mentha haplocalyx with NaN3 was preliminarily established.
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