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OMIP-048 MC: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry
Agnieszka Jaracz-Ros1, Patrice Hémon1, Roman Krzysiek1
1Inflammation Chimiokines et Immunopathologie, INSERM, Faculté de Médecine, Universite Paris-Sud, Université Paris-Saclay, Clamart, France.
Abstract:
Calcium (Ca2+ ) signaling controls T-cell activation and functions. Ca2+ concentrations are locally detected and controlled by Ca2+ -sensors (STIM1 and 2 detecting the depletion from ER stores channels) and Ca2+ -channels (ORAI1-3 in the cell membrane and VDAC1 in the outer mitochondrial membrane). We first validated and titrated antibodies to assess the expression of these Ca2+ -sensors and -channels in human and murine cells, and further devised a 18-antibodies mass cytometry panel to characterize their expression in primary murine lymphocyte subsets.
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