Related Experiment Video
Updated: Feb 7, 2026

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry
Published on: February 2, 2024
[Study on Chemical Constituents of Delphinium caeruleum]
Objective:
To study the chemical constituents from the whole plant of Delphinium caeruleum.
Methods:
The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures of the isolated compounds were elucidated by spectroscopic analysis and physicochemical properties.
Results:
Twelve compounds were isolated and purified from the ethanol extract of Delphinium caeruleum. They were identified as β-sitosterol( 1),kaempferol( 2),quercetin( 3),isovanillic acid( 4),apigenin( 5),luteolin( 6),8-methoxy-5,7,3’,4’-tetrahydroxy-flavone( 7),β-daucosterol( 8),kaempferol-3-O-β-D-glucoside( 9),3,5-dihydroxy-4’-methoxyflavone-7-yl-O-β-D-glucopyranosyl-( 1→4)-α-L-rhamnopyranoside( 10),rutin( 11) and sucrose( 12).
Conclusion:
Compounds 1 ~ 12 are isolated from this plant for the first time.
Related Concept Videos
Experimental Determination of Chemical Formula
Chemical Formulas
Chemical Equations
Chemical Reactions
Chemical Reactions Rearrange Atoms into New Substances
A chemical reaction takes starting materials—the reactants—and changes them...
Chemical Reactions
The relative amounts of reactants and products represented in a balanced chemical equation are often referred to as stoichiometric amounts. However, in...
Types of Chemical Bonds

