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[Study on Anti-RSV Activities and QSAR of Natural Caffeoylquinic Acid Derivates]
Objective:
To study the inhibitory effects of natural caffeoylquinic acid derivates against respiratory syncytial virus( RSV) in vitro and their quantitative structure-activity relationship.
Methods:
The anti-RSV activities of caffeoylquinic acid derivates were detected by cytopathic effect( CPE) method,MTT assay was adopted to evaluate the cytotoxicities of caffeoylquinic acid derivates and a three-dimensional quantitative structure-activity relationship( 3D-QSAR) model was built.
Results:
The caffeoylquinic acid derivates showed different levels of anti-RSV activity. 4,5-d-CQAME( CQA-4) was the most effective anti-RSV compound with IC50 of 2. 5μmol /L,and the SI was greater than 153. 8. Its effect was much better than the positive control drug ribavirin with the IC50 of 8. 3 μmol /L,and SI > 20,respectively. The result of 3D-QSAR study showed that caffeoyl group was important for the anti-RSV activity.
Conclusion:
Natural caffeoylquinic acid derivates exhibit anti-RSV activity and dicaffeoylquinic derivates are the most effective anti-RSV compounds. Both configuration and substituent groups have a great influence on the anti-RSV activity.
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