Related Experiment Video
Updated: Feb 7, 2026

Microtiter Dish Biofilm Formation Assay
Published on: January 30, 2011
New Thiazole Nortopsentin Analogues Inhibit Bacterial Biofilm Formation
Anna Carbone1, Barbara Parrino2, Maria Grazia Cusimano3
1Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90100 Palermo, Italy. anna.carbone@unipa.it.
Abstract:
New thiazole nortopsentin analogues were conveniently synthesized and evaluated for their activity as inhibitors of biofilm formation of relevant Gram-positive and Gram-negative pathogens. All compounds were able to interfere with the first step of biofilm formation in a dose-dependent manner, showing a selectivity against the staphylococcal strains. The most active derivatives elicited IC50 values against Staphylococcus aureus ATCC 25923, ranging from 0.40⁻2.03 µM. The new compounds showed a typical anti-virulence profile, being able to inhibit the biofilm formation without affecting the microbial growth in the planktonic form.
Related Concept Videos
Bacterial Signaling
Feedback Inhibition
Biofilms
Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Enzyme Inhibition
Inhibition of Cdk Activity

