Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Statistical Significance01:50

Statistical Significance

22.0K
Once data is collected from both the experimental and the control groups, a statistical analysis is conducted to find out if there are meaningful differences between the two groups. A statistical analysis determines how likely any difference found is due to chance (and thus not meaningful). In psychology, group differences are considered meaningful, or significant, if the odds that these differences occurred by chance alone are 5 percent or less. Stated another way, if we repeated this...
22.0K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.4K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.4K
Fundamental Attribution Error01:14

Fundamental Attribution Error

13.8K
According to some social psychologists, people tend to overemphasize internal factors as explanations—or attributions—for the behavior of other people. They tend to assume that the behavior of another person is a trait of that person, and to underestimate the power of the situation on the behavior of others. They tend to fail to recognize when the behavior of another is due to situational variables, and thus to the person’s state. This erroneous assumption is...
13.8K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

843
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
843
Poisson's Ratio01:23

Poisson's Ratio

1.2K
Poisson's ratio is a material property that indicates their stress response. It explains the connection between the elongation or compression a material undergoes in the direction of an applied force and the contraction or expansion it experiences perpendicular to that force. When a slender bar is loaded axially, it stretches in the direction of the force and contracts laterally. Poisson's ratio is the negative ratio of this lateral contraction to the axial elongation. The negative sign...
1.2K
Poisson Probability Distribution01:09

Poisson Probability Distribution

12.1K
A Poisson probability distribution is a discrete probability distribution. It gives the probability of a number of events occurring in a fixed interval of time or space if these events happen at a known average rate and independently of the time since the last event. For example, a book editor might be interested in the number of words spelled incorrectly in a particular book. It might be that, on average, there are five words spelled incorrectly in 100 pages. The interval is 100 pages.
The...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-speed volumetric single-molecule imaging using dual-wavelength light sheets and PSF-engineered enhanced biplane detection.

bioRxiv : the preprint server for biology·2026
Same author

Photoswitchable Radicals as Reporter Spins for Quantum Sensing with Spin Defects in Diamond.

Journal of the American Chemical Society·2026
Same author

Quantum-inspired super-resolution of fluorescent point-like sources.

Nature communications·2026
Same author

Compact Spectral Encoding Microscopy by Terrace Grating Optics.

ACS photonics·2026
Same author

Quantifying classical and quantum bounds for resolving closely spaced, non-interacting, simultaneously emitting dipole sources in optical microscopy.

The Journal of chemical physics·2026
Same author

Platelet and Fibrinogen Dynamics After CAR-T Cell Therapy in Relapsed/Refractory B-Cell Lymphoma May Predict ICANS Onset.

European journal of haematology·2026

Related Experiment Video

Updated: Feb 6, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

18.9K

Fundamental Precision Bounds for Three-Dimensional Optical Localization Microscopy with Poisson Statistics.

Mikael P Backlund1,2, Yoav Shechtman3, Ronald L Walsworth1,2

  • 1Harvard-Smithsonian Center for Astrophysics, Cambridge, Massachusetts 02138, USA.

Physical Review Letters
|August 8, 2018
PubMed
Summary

Researchers analyzed optical imaging depth localization methods, finding some exceed quantum limits. An interferometer design approaches these limits, offering insights for advanced microscopy and quantum estimation.

More Related Videos

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

11.2K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

10.7K

Related Experiment Videos

Last Updated: Feb 6, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

18.9K
Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

11.2K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

Published on: October 29, 2019

10.7K

Area of Science:

  • Optical Imaging and Microscopy
  • Quantum Metrology
  • Biophysics

Background:

  • Precise three-dimensional localization of single fluorophores is crucial for biological microscopy.
  • Emitter depth localization is significantly more challenging than lateral localization in optical imaging.
  • Existing microscopy techniques engineer optical responses to enhance depth information.

Purpose of the Study:

  • To evaluate the optimality of current single-objective depth localization methods in optical microscopy.
  • To derive and compare existing methods against the measurement-independent quantum Cramér-Rao bound (QCRB).
  • To propose novel interferometer arrangements for improved depth localization and quantum parameter estimation.

Main Methods:

  • Theoretical derivation and comparison of microscopy localization methods to the quantum Cramér-Rao bound (QCRB).
  • Analysis of single-objective collection strategies for emitter depth localization.
  • Proposal and analysis of an interferometer arrangement for enhanced depth information acquisition.

Main Results:

  • Existing single-objective depth localization methods in microscopy exceed the quantum Cramér-Rao bound (QCRB).
  • A proposed interferometer arrangement demonstrates near-optimal depth localization performance, approaching the QCRB.
  • An established interferometric technique with two opposed objectives achieves the QCRB in all three dimensions simultaneously.

Conclusions:

  • Current single-objective microscopy approaches for depth localization are suboptimal.
  • Interferometric techniques offer a promising avenue for achieving quantum-limited precision in 3D localization.
  • The findings provide a valuable case study for quantum multiparameter estimation in optical imaging.