Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Coated vesicles: characterization, selective dissociation, and reassembly.

M P Woodward, T F Roth

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Process implemented for using nursing research in practice.

    Oncology nursing forum·1992
    Same author

    The antigenic surface of staphylococcal nuclease. I. Mapping epitopes by site-directed mutagenesis.

    Journal of immunology (Baltimore, Md. : 1950)·1991
    Same author

    Cultured chick yolk sac epithelium: structure and IgG surface binding.

    European journal of cell biology·1990
    Same author

    Membranous intermediates in endocytosis are labile, as shown in a temperature-sensitive mutant.

    Proceedings of the National Academy of Sciences of the United States of America·1989
    Same author

    Purification of chicken liver ferritin by two novel methods and structural comparison with horse spleen ferritin.

    The Biochemical journal·1989
    Same author

    Coated vesicles from chicken liver bind ferritin.

    Journal of cell science·1989
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Researchers isolated major proteins from porcine brain coated vesicles, identifying clathrin as a key component. They found clathrin can be solubilized and reassembled into coat structures under specific conditions.

    Area of Science:

    • Cell Biology
    • Biochemistry

    Background:

    • Coated vesicles are essential for intracellular transport.
    • Understanding their protein composition is crucial for elucidating their function.

    Purpose of the Study:

    • To identify and characterize the major proteins of porcine brain coated vesicles.
    • To investigate the conditions under which clathrin, a major coat protein, can be solubilized and reassembled.

    Main Methods:

    • Sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze protein composition.
    • Coated vesicles and their coats were isolated from porcine brain.
    • Protein solubilization and reassembly were induced using urea, MgCl2, and pH changes.

    Main Results:

    Related Experiment Videos

  • Three major proteins (180,000, 125,000, and 55,000 daltons) constituted 73% of coated vesicle protein.
  • Clathrin (180,000 daltons) comprised 40% of the total coated vesicle protein.
  • Clathrin was solubilized by 2 M urea, 0.25 M MgCl2, or pH 7.5 and could be reassembled into coat structures.

    • Conclusions:

    • Clathrin is the predominant protein in porcine brain coated vesicle coats.
    • Clathrin's ability to disassemble and reassemble suggests a dynamic role in vesicle formation and transport.