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Updated: Feb 6, 2026
08:51
Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
Published on: June 20, 2025
658
A carbon nanoparticles-based solid-phase purification method facilitating sensitive MALDI-MS analysis of
Jieqiang Zhong1, Alireza Banazadeh1, Wenjing Peng1
1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Electrophoresis
|August 8, 2018
Summary
A new carbon nanoparticles (CNPs) purification method enhances direct analysis of permethylated glycans using MALDI-MS. This technique offers high recovery rates and potential for improved glycan biomarker discovery in diseases.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Materials Science
Background:
- Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is crucial for glycan analysis.
- Native glycans exhibit low ionization efficiency, hindering direct MS analysis.
- Permethylation improves ionization but introduces salt, suppressing MS signals, necessitating purification.
Purpose of the Study:
- To develop a novel solid-phase purification method for permethylated glycans.
- To enable direct MALDI-MS analysis of purified permethylated glycans.
- To evaluate carbon nanoparticles (CNPs) as an effective purification sorbent.
Main Methods:
- Investigated carbon nanoparticles (CNPs), graphene nanosheets (GNs), activated charcoal, and porous graphitic carbon (PGC) as sorbents.
- Purified permethylated N-glycans from ribonuclease B and fetuin.
- Applied the CNPs-based method to glycans from human sera across esophageal disease stages.
Main Results:
- CNPs demonstrated superior purification performance compared to other carbon materials.
- The method achieved over 97% recovery rate, outperforming online purification.
- Identified distinct glycan structures and significantly different relative intensities in sera from patients with adenocarcinoma and Barret's esophagus.
Conclusions:
- CNPs offer a highly effective and efficient solid-phase purification method for permethylated glycans.
- This approach facilitates direct MALDI-MS analysis with high glycan recovery.
- CNPs show promise as a superior alternative to existing purification sorbents for glycomic studies and disease biomarker discovery.
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