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A Green BODIPY-Based, Super-Fluorogenic, Protein-Specific Labelling Agent.

Yingche Chen1, Kelvin Tsao1, Sydney L Acton1

  • 1Department of Chemistry, University of Ottawa, 10 Marie-Curie, Ottawa, ON, K1N 6N5, Canada.

Angewandte Chemie (International Ed. in English)
|August 8, 2018
PubMed
Summary
This summary is machine-generated.

We developed YC23, a novel green probe, for fluorogenic labeling of proteins. This method works in bacterial lysates and living mammalian cells using a specific peptide tag.

Keywords:
BODIPYfluorogenic probesmaleimideprotein labelling

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Area of Science:

  • Chemical Biology
  • Biochemistry
  • Molecular Imaging

Background:

  • Protein labeling is crucial for biological research.
  • Existing methods may have limitations in specificity or application.
  • Development of novel probes is needed for advanced biological studies.

Purpose of the Study:

  • To develop a novel, green fluorescent probe for protein labeling.
  • To establish a fluorogenic addition reaction (FlARe) for specific protein tagging.
  • To demonstrate the utility of this system in bacterial lysate and live cells.

Main Methods:

  • Synthesized YC23, a BODIPY-based dimaleimide derivative.
  • Developed a genetically encodable peptide tag (dC10α).
  • Utilized the fluorogenic addition reaction (FlARe) between YC23 and dC10α for labeling.

Main Results:

  • YC23 demonstrated efficient fluorogenic labeling via FlARe with the dC10α tag.
  • Specific labeling of a protein of interest (POI) was achieved in bacterial lysate.
  • Successful fluorogenic labeling of a POI was demonstrated in living mammalian cells.

Conclusions:

  • YC23 and the FlARe reaction provide a novel and effective method for specific protein labeling.
  • This system is applicable in cell-free systems and in vivo, offering versatility.
  • The developed probe and reaction advance capabilities in chemical biology and molecular imaging.