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Updated: May 16, 2025

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
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Methods for kinetic evaluation of reversible covalent inhibitors from time-dependent IC50 data

Lavleen K Mader1, Jeffrey W Keillor1

  • 1Department of Chemistry and Biomolecular Sciences, University of Ottawa Ottawa Ontario K1N 6N5 Canada jkeillor@uottawa.ca.

RSC Medicinal Chemistry
|March 31, 2025
PubMed
Summary

New methods quantify time-dependent inhibition kinetics for reversible covalent drugs. These tools help accurately rank drug candidates by analyzing incubation time-dependent IC50 data for better drug development.

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