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An investigation on reduction process of cucumber ascorbate oxidase
Biochemical and Biophysical Research Communications
|March 13, 1986
Summary
Cucumber ascorbate oxidase reduction by L-ascorbate was studied. Specific copper types within the enzyme
Area of Science:
- Biochemistry
- Enzymology
- Spectroscopy
Background:
- Ascorbate oxidase is a key enzyme in plant metabolism.
- Understanding its active site and reduction mechanisms is crucial.
Purpose of the Study:
- To investigate the reduction process of cucumber ascorbate oxidase.
- To elucidate the roles of different copper centers during reduction.
Main Methods:
- Anaerobic absorption spectroscopy.
- Electron paramagnetic resonance (EPR) spectroscopy.
Main Results:
- One type I copper and a pair of type III coppers reduced faster.
- The enzyme's active site comprises one type I, one type II, and a pair of type III coppers.
- Type II copper reduction occurred after type I and type III coppers.
Conclusions:
- Differential reduction rates of copper centers were observed.
- The active site structure and copper coordination influence the reduction pathway.