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A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources
10:24

A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources

Published on: January 27, 2026

311

Effect of polymyxin-B on T-lymphocyte protein synthesis

B S Baliga, L J Sindel, L D Jenkins

    Biochemical and Biophysical Research Communications
    |March 13, 1986
    PubMed

    Insights

    Protein kinase-C (PK-C) activity is crucial for T-lymphocyte activation. Inhibiting PK-C with polymyxin-B significantly reduced protein and DNA synthesis in T-cells, highlighting PK-C

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • T-lymphocytes play a vital role in immune responses.
    • Mitogen-activated T-lymphocytes undergo significant cellular changes, including protein and DNA synthesis.
    • Protein kinase-C (PK-C) is implicated in cellular signaling pathways.

    Purpose of the Study:

    • To investigate the role of protein kinase-C (PK-C) phosphorylation in mitogen-induced T-lymphocyte responses.
    • To determine the effect of PK-C inhibition on T-lymphocyte activation markers.

    Main Methods:

    • T-lymphocytes were activated using phytohemagglutinin (PHA).
    • The effect of polymyxin-B, a PK-C inhibitor, on [3H]-thymidine and [3H]-leucine incorporation was measured.
    • PK-C phosphorylating activity was assessed in polymyxin-B treated and untreated T-lymphocytes.

    Main Results:

    • Polymyxin-B significantly inhibited PHA-induced [3H]-thymidine incorporation in T-lymphocytes.
    • A dose-dependent inhibition of [3H]-leucine incorporation was observed with polymyxin-B treatment.
    • PK-C activity was reduced by over 75% in T-lymphocytes treated with polymyxin-B.

    Conclusions:

    • Protein kinase-C (PK-C) activity is essential for T-lymphocyte activation.
    • PK-C phosphorylation is a key regulatory step in mitogen-induced protein synthesis in T-lymphocytes.
    • Inhibition of PK-C impairs T-lymphocyte proliferation and protein synthesis.

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