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Related Experiment Videos

Sequential antibody changes following ulcerative herpetic keratitis.

K R Wilhelmus, S Darougar, T Forsey

    The British Journal of Ophthalmology
    |May 1, 1986
    PubMed
    Summary

    This study on herpes simplex virus (HSV) dendritic keratitis found tear IgG may originate from blood. Laboratory testing guidelines for recurrent herpetic keratitis are provided.

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    Transactions of the American Ophthalmological Society·2002

    Area of Science:

    • Ophthalmology
    • Virology
    • Immunology

    Background:

    • Herpes simplex virus (HSV) is a common cause of infectious keratitis.
    • Recurrent herpetic keratitis presents diagnostic challenges.
    • Understanding immunoglobulin profiles in ocular infections is crucial.

    Purpose of the Study:

    • To investigate immunoglobulin levels (IgG, IgM, IgA) against HSV in blood and tears of patients with dendritic keratitis.
    • To determine the origin of tear immunoglobulins.
    • To provide insights for laboratory diagnostics in recurrent herpetic keratitis.

    Main Methods:

    • Indirect micro-immunofluorescent technique used to detect HSV-specific IgG, IgM, and IgA.
    • Blood and tear samples collected from 28 patients over 28 days.
    • Corneal and conjunctival swabbings for HSV isolation.

    Main Results:

    • HSV IgG was detected in blood and tears; circulating HSV IgM and local HSV IgA were not found.
    • Most patients showed non-diagnostic fluctuations in HSV IgG over four weeks, with 25% exhibiting a rising blood IgG titre.
    • Tear IgG is suggested to be an exudate from blood.
    • HSV was isolated from 68% of corneal and 11% of conjunctival samples.

    Conclusions:

    • Tear IgG is likely derived from blood in herpetic keratitis patients.
    • The study offers guidance for laboratory testing strategies in recurrent herpetic keratitis.
    • Further research may refine diagnostic approaches for HSV ocular infections.

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