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Updated: Feb 6, 2026
10:37
Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
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Fine-Tuning Mybl2 Is Required for Proper Mesenchymal-to-Epithelial Transition during Somatic Reprogramming
Carl Ward1, Giacomo Volpe1, Pierre Cauchy2
1Institute of Cancer and Genomic Science, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
Cell Reports
|August 9, 2018
Summary
Overexpression of Mybl2 inhibits somatic reprogramming by altering chromatin accessibility, blocking key gene activation. This finding reveals Mybl2 as a critical gatekeeper for initiating cell fate changes during reprogramming.
Area of Science:
- Cell biology
- Epigenetics
- Molecular biology
Background:
- Somatic reprogramming involves Yamanaka factors activating pluripotency networks.
- Understanding factors that regulate reprogramming dynamics is crucial for cell fate control.
Purpose of the Study:
- To investigate the role of Mybl2 in somatic reprogramming.
- To elucidate the molecular mechanisms by which Mybl2 influences reprogramming initiation.
Main Methods:
- Overexpression of Mybl2 in somatic cells.
- Chromatin conformation analysis.
- Gene expression profiling to assess reprogramming markers and immediate early response genes.
Main Results:
- Mybl2 overexpression significantly inhibits somatic reprogramming.
- Mybl2 alters chromatin conformation, impacting pioneer factor accessibility.
- Mybl2 promotes accessibility for reprogramming-blocking early immediate response genes.
- Chromatin landscape changes induced by Mybl2 lead to deregulation of mesenchymal-to-epithelial transition genes.
Conclusions:
- Mybl2 acts as a gatekeeper for initiating somatic reprogramming.
- Mybl2 levels are critical for regulating the dynamics of the reprogramming process.
- This study provides insights into the coordination of molecular events required for cell fate changes.
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