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Related Experiment Video

Updated: Feb 6, 2026

High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping

High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping

Published on: June 29, 2018

Combining genotypic and phenotypic analyses on single mutant zebrafish larvae.

Barbara Dupret^1,2, Pamela Völkel^1,2,3, Pauline Follet^1,2,4,5

¹Inserm U908, Cell Plasticity & Cancer, Lille, France.

|August 10, 2018

Summary

This study introduces a novel method for analyzing zebrafish mutants, enabling simultaneous genotyping and phenotypic studies on single larvae. This technique overcomes challenges posed by homozygous lethal mutations, advancing zebrafish research.

Keywords:

Genotyping Histology Histone extraction Protein extraction RNA extraction Spinal cord regeneration Zebrafish

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Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing

Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing

Published on: September 1, 2017

Area of Science:

Developmental biology
Genetics
Animal models

Background:

Zebrafish are crucial for studying vertebrate development and disease.
Genome editing technologies like CRISPR/Cas9 have increased zebrafish mutant generation.
Homozygous lethal mutations hinder the creation of complete mutant lines.

Purpose of the Study:

To present a method for simultaneous genotyping and phenotypic analysis of zebrafish mutants.
To enable research on homozygous lethal mutations.
To optimize protocols for single zebrafish embryos and larvae.

Main Methods:

Larval tail transection for genotyping.
Phenotypic analysis of the anterior larval part.
Protocols for histology, protein/histone extraction (Western blot), RNA extraction (RT-PCR), and spinal cord regeneration studies.

Main Results:

A streamlined procedure for analyzing mutant zebrafish larvae from heterozygous incrosses.
Successful application of the method on single embryos and larvae.
Facilitation of comprehensive studies despite homozygous lethality.

Conclusions:

The presented method enhances the utility of zebrafish as a model organism.
It allows for detailed characterization of mutants with embryonic or larval lethal phenotypes.
This technique supports advanced genetic and developmental studies in zebrafish.