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Related Concept Videos

Next-generation Sequencing03:00

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Parallel Resonance01:23

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The parallel RLC circuit is an arrangement where the resistor (R), inductor (L), and capacitor (C) are all connected to the same nodes and, as a result, share the same voltage across them. The parallel RLC circuit is analyzed in terms of admittance (Y), which reflects the ease with which current can flow. The admittance is given by:
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Parallel Processing01:20

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The brain processes sensory information rapidly due to parallel processing, which involves sending data across multiple neural pathways at the same time. This method allows the brain to manage various sensory qualities, such as shapes, colors, movements, and locations, all concurrently. For instance, when observing a forest landscape, the brain simultaneously processes the movement of leaves, the shapes of trees, the depth between them, and the various shades of green. This enables a quick and...
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Resistors are in parallel when one end of all the resistors are connected to a continuous wire of negligible resistance and the other end of all the resistors are also connected to one another through a continuous wire of negligible resistance. In the case of a parallel configuration, the potential drop across each resistor is the same. Current through each resistor can be found using Ohm’s law, I = V/R, where the voltage is constant across each resistor. The sum of the individual currents...
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Series and Parallel Capacitors01:14

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Capacitors, fundamental components in electronic circuits, can be connected in series and/or parallel configurations. Each configuration has different impacts on the overall behavior of the circuit.
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The parallel-axis theorem provides a convenient and quick method of finding the moment of inertia of an object about an axis parallel to the axis passing through its center of mass. Consider a thin rod as an example. There is a striking similarity between the process of finding the moment of inertia of a thin rod about an axis through its middle, where the center of mass lies, and about an axis through its end using the conventional method. In the conventional method, the concept of linear mass...
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Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
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Massively parallel and multiplex blood group genotyping using next-generation-sequencing.

Stéphanie A Boccoz1, Julien Fouret2, Magali Roche3

  • 1AXO Science SAS, 66 Bd Niels Bohr CEI 1, 69100 Villeurbanne, France.

Clinical Biochemistry
|August 10, 2018
PubMed
Summary
This summary is machine-generated.

A new Next-Generation Sequencing (NGS) assay accurately genotypes blood group single nucleotide polymorphisms (SNPs) directly from whole blood. This method offers a faster, more comprehensive alternative to traditional blood typing techniques.

Keywords:
Blood group genotypingMultiplexNGSSNP

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Area of Science:

  • Genetics and Genomics
  • Transfusion Medicine
  • Molecular Diagnostics

Background:

  • Over 350 blood group antigens exist, primarily determined by single nucleotide polymorphisms (SNPs).
  • Current serological blood typing methods have limitations in handling the vast number of antigens and growing demands.
  • There is a need for advanced techniques to efficiently and accurately characterize blood group systems.

Purpose of the Study:

  • To develop and evaluate a Next-Generation Sequencing (NGS) assay for simultaneous blood group genotyping.
  • To assess the assay's performance in identifying 15 SNPs across multiple blood group systems from whole blood samples.
  • To compare the NGS results with established blood group typing methods.

Main Methods:

  • Automated DNA extraction followed by multiplex polymerase chain reaction (PCRm) amplification of target SNPs.
  • Two custom panels were designed to cover 9 blood group systems and 15 SNPs.
  • Dual-indexed library preparation and sequencing on the Illumina MiSeq platform.

Main Results:

  • The NGS assay successfully genotyped 95 blood donor samples in a single run.
  • High accuracy was achieved, identifying 1420 out of 1425 targeted SNPs (99.65% coverage).
  • Excellent correlation (99%) was observed between NGS genotyping and conventional blood group typing methods.

Conclusions:

  • Next-Generation Sequencing (NGS) provides a powerful tool to supplement and potentially replace existing serological and molecular blood typing techniques.
  • The developed NGS assay offers complete and rapid results for pre-screening and identifying rare blood types.
  • This technology has the potential to significantly advance transfusion medicine practices.