Related Experiment Video
Updated: Feb 6, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
Published on: December 5, 2016
Quantification of methylation efficiency at a specific N6-methyladenosine position in rRNA by using BNA probes
Takuya Oshima1, Kensuke Ishiguro, Tsutomu Suzuki
1Department of RNA Biology and Neuroscience, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan. ykawahara@rna.med.osaka-u.ac.jp.
Abstract:
We found that insertion of artificial nucleic acid analogs, such as bridged nucleic acid (BNA), into DNA probes increases the difference in melting temperature between N6-methyladenosine (m6A)-containing RNA and unmethylated RNA. By applying this principle, we quantified methylation efficiency at m6A sites in E. coli 23S rRNA with high accuracy.
Related Concept Videos
Position-effect Variegation
Positive Regulator Molecules
Position and Displacement
Serial Position Effect
Chromatin Position Affects Gene Expression
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Position Vectors
For instance, we want to locate a point P(x, y, z) relative to the origin of coordinates O. In that case, we can define a position...

