Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10

The complete sequence of the RNA segment that codes for a major outer capsid protein (VP5) of bluetongue virus serotype 10 has been determined from overlapping cDNA clones inserted into pBR322. The segment 5 RNA of the virus (M5 RNA) is deduced to be 1638 base pairs long (1.05 X 10(6) daltons) and has an open reading frame in one strand capable of coding for a protein with a calculated size of 59 163 daltons (526 amino acids) and a net charge of -4.5 at neutral pH.