Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

14.7K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.7K
Protein and Protein Structure02:15

Protein and Protein Structure

88.2K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
88.2K
Simple Staining Technique01:24

Simple Staining Technique

3.8K
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
3.8K
Differential Staining Technique01:26

Differential Staining Technique

2.3K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
2.3K
Special Staining Techniques01:13

Special Staining Techniques

1.3K
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
1.3K
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.1K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amytrapper Catheter: A Prototype Extracorporeal Device That Traps Blood Amyloid-β in a Rat Model of Alzheimer's Disease.

American journal of Alzheimer's disease and other dementias·2025
Same author

Novel API Coated Catheter Removes Amyloid-β from Plasma of Patients with Alzheimer's Disease.

HSOA journal of alzheimer's & neurodegenerative diseases·2021
Same author

Effect of AmyTrap, an amyloid-β binding drug, on Aβ induced mitochondrial dysfunction and tau phosphorylation in cultured neuroblastoma cells.

Metabolic brain disease·2020
Same author

Pre-Clinical Safety and Efficacy Evaluation of Amytrap, a Novel Therapeutic to Treat Alzheimer's Disease.

Journal of Alzheimer's disease reports·2019
Same author

'Amytrapper', a Novel Immobilized Sepharose API Matrix, Removes Amyloid-β from Circulation <i>in vitro</i>.

Journal of Alzheimer's disease reports·2019
Same author

Patents on potential drugs to treat Alzheimer's disease: special emphasis on small peptides.

Recent patents on CNS drug discovery·2014
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 6, 2026

Staining Proteins in Gels
10:55

Staining Proteins in Gels

Published on: July 8, 2008

21.2K

Protein Stains and Applications.

Pazhani Sundaram1

  • 1Recombinant Technologies LLC, 1090 Meriden Waterbury Turnpike, Suite 1, Cheshire, CT 06410, USA. recombtech@yahoo.com.

Methods in Molecular Biology (Clifton, N.J.)
|August 12, 2018
PubMed
Summary
This summary is machine-generated.

This review covers protein staining methods used in gel electrophoresis to determine biopolymer properties. It details various stains, their applications, and imaging considerations for accurate protein detection.

Keywords:
2-DECoomassie Brilliant BlueFluorescent stainsPreelectrophoresis stainingSDS-PAGESilver nitrateZinc staining

More Related Videos

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
07:47

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Published on: August 14, 2009

54.8K
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

15.1K

Related Experiment Videos

Last Updated: Feb 6, 2026

Staining Proteins in Gels
10:55

Staining Proteins in Gels

Published on: July 8, 2008

21.2K
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
07:47

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Published on: August 14, 2009

54.8K
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

15.1K

Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry

Background:

  • Protein staining is essential for analyzing biopolymers like proteins after separation by gel electrophoresis.
  • Various protein stains exist, differing in sensitivity, ease of use, color, stability, versatility, and specificity.

Purpose of the Study:

  • To review and compare different protein staining techniques for gels and blots.
  • To provide guidance on stain usage, advantages, disadvantages, and imaging considerations.
  • To discuss stains for general protein detection and post-translational modification-specific detection.

Main Methods:

  • Literature review of protein staining methods.
  • Comparative analysis of stain properties (sensitivity, specificity, etc.).
  • Discussion of application protocols and imaging techniques.

Main Results:

  • Detailed comparison of common colorimetric and fluorescent protein stains.
  • Evaluation of stains for general protein detection and specialized applications.
  • Identification of key factors for effective protein imaging and data interpretation.

Conclusions:

  • The choice of protein stain significantly impacts the accuracy of biopolymer analysis.
  • Understanding stain characteristics is crucial for selecting the optimal method for specific research needs.
  • This review serves as a comprehensive guide for researchers utilizing protein staining techniques.