Related Experiment Video
Updated: Feb 6, 2026

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection
Published on: December 19, 2025
Multicolored Prestained Standard Protein Marker Generation Using a Variety of Remazol Dyes for Easy Visualization of
1Oklahoma Medical Research Foundation, University of Oklahoma, Oklahoma City, OK, USA. gaurav-kumar@omrf.org.
Abstract:
Isolation and detection of a specific protein from a complex mixture of proteins using molecular sieves provided by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique in molecular biology and biochemistry. The detection is achieved usually by running prestained standard markers along with the sample protein mixture. Multicolored standard markers are preferred compared to fluorescent labeled or single colored markers as it allows easy visualization of separation of a specific protein band during SDS-PAGE. In addition, these colored markers show evidence for the transfer of protein bands on the membrane in western blotting technique. This review describes a very simple, inexpensive and easy method of prestaining specific proteins with different colors using a variety of Remazol dyes.
Related Concept Videos
Protein-protein Interfaces
Band Theory
The energy difference between these bands is known as the band gap.
Conductor, Semiconductor,...
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
Protein and Protein Structure
A protein's shape is critical to its function. For example, an enzyme...
Protein Digestion
Conjugated Proteins
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

