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Detection of Phosphoproteins in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Using 8-Quinolinol Stain
Sun-Young Hwang1, Xu Wang1,2, Jung-Kap Choi3
1Laboratory of Analytical Biochemistry, College of Pharmacy & Research Institute of Drug Development, Chonnam National University, Gwangju, South Korea.
Abstract:
In order to detect phosphoproteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), an easy and fast fluorescent detection method is described. 8-Quinolinol can form ternary complexes in the gel matrix contributed by the affinity of aluminum ion to the phosphate groups on the proteins and the metal chelating property of 8-Quinolinol, exhibiting strong fluorescence in ultraviolet light. It can visualize as little as 4-8 ng of α-casein and β-casein, 15-31 ng of ovalbumin and κ-casein within 70 min. The approach utilizing 8-quinolinol could be an alternative staining method for phosphoproteomics.
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