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Related Concept Videos

Dry Friction01:30

Dry Friction

996
Dry friction occurs between two solid surfaces in contact as they attempt to move relative to one another. In daily life, dry friction is encountered in various forms, such as when walking on the ground, sliding an object across a table, or rubbing hands together. Despite its ubiquity, the underlying mechanisms behind dry friction are not readily visible.
To illustrate this concept, imagine a wooden crate resting on a rough, non-uniform horizontal surface. When an external force is applied to...
996
Drying Shrinkage01:21

Drying Shrinkage

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When hardened concrete is exposed to air with a relative humidity of less than 100 percent, it begins to lose the free water within its capillaries. As this water evaporates, the water initially adsorbed onto the calcium silicate hydrates migrates towards these now empty spaces and eventually evaporates as well. Over time, as more water leaves, the volume of the concrete decreases, a phenomenon known as drying shrinkage.
A portion of this drying shrinkage can be reversed; if the concrete is...
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Characteristics of Dry Friction01:21

Characteristics of Dry Friction

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Dry friction occurs when two solid surfaces slide against each other without any lubrication or fluid present. It causes resistance when pushing objects along a surface, like a gardener pushing a wheelbarrow. The force applied to move the cart causes dry friction between the wheel and the ground.
Before the wheelbarrow starts moving, the static frictional force acts tangentially to the contact surface, opposing the force that is about to induce the motion. This frictional force prevents the...
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Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
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Washing, Drying, and Ignition of Precipitates00:52

Washing, Drying, and Ignition of Precipitates

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After filtration, the precipitate is washed to remove coprecipitated impurities and any remaining mother liquor. Colloidal precipitates, such as silver chloride, are washed with an electrolyte (such as dilute nitric acid) to prevent the peptization of the precipitate. In the case of slightly soluble precipitates, the wash solution contains a common ion to reduce solubility. Lead sulfate, which is slightly soluble in water, is washed with dilute sulfuric acid. Similarly, wash solutions may be...
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DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

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Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
08:31

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

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Gel Drying Methods.

Anja Feldmann1, Nicole Berndt1, Ralf Bergmann1

  • 1Institute of Radiopharmaceutical Cancer Research, Radioimmunology, Helmholtz-Zentrum Dresden-Rossendorf e.V. (HZDR), Dresden, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 12, 2018
PubMed
Summary
This summary is machine-generated.

Drying polyacrylamide gels after electrophoresis is essential for autoradiography or storage. A simple, inexpensive protocol is presented as an alternative to commercial methods, ideal for lab book archiving.

Keywords:
Gel dryingPolyacrylamide gelsProteins

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Polyacrylamide gel electrophoresis (PAGE) is a fundamental technique in molecular biology.
  • Dried gels are required for specific downstream applications like autoradiography.
  • Existing commercial drying methods can be expensive and laborious.

Purpose of the Study:

  • To present a simple and cost-effective method for drying polyacrylamide gels.
  • To provide an alternative to commercial gel drying solutions.
  • To facilitate the archival storage of gels.

Main Methods:

  • The study details a straightforward protocol for drying polyacrylamide gels.
  • The method is designed to be easily implemented in a standard laboratory setting.

Main Results:

  • The presented protocol effectively dries polyacrylamide gels.
  • The dried gels are suitable for storage in laboratory notebooks.
  • The method avoids the complexity and cost of commercial alternatives.

Conclusions:

  • A simple and economical gel drying protocol is described.
  • This method is particularly useful for preserving gels for long-term storage.
  • The protocol offers a practical solution for researchers needing to archive electrophoresis gels.