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Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

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Published on: May 6, 2018

Bile salt hydrolases: Structure and function, substrate preference, and inhibitor development.

Zixing Dong¹, Byong H Lee^2,3

¹College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin, 300457, China.

Protein Science : a Publication of the Protein Society

|August 12, 2018

Summary

This summary is machine-generated.

New research explores bile salt hydrolase (BSH) inhibitors as alternatives to antibiotic growth promoters (AGPs) in animal feed. Understanding BSH structure and function is key to developing effective, non-antibiotic solutions for animal performance.

Keywords:

BSH inhibitors animal feed supplements antibiotic growth promoters bile salt hydrolase fat digestion structural basis for the substrate preference

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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

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Area of Science:

Biochemistry
Animal Nutrition
Enzymology

Background:

The global reduction of antibiotic growth promoters (AGPs) in animal production necessitates alternative strategies for enhancing animal performance.
Bile salt hydrolase (BSH) activity in the gut is linked to reduced growth promotion by AGPs, suggesting BSH as a therapeutic target.
BSH negatively impacts host fat digestion and energy absorption, making its inhibition a promising avenue.

Purpose of the Study:

To review the structure-function relationships of BSH enzymes.
To explore the molecular basis of BSH substrate recognition.
To discuss the development of BSH inhibitors as alternatives to AGPs.

Main Methods:

Analysis of crystal structures of BSH enzymes.
Review of kinetic data for BSH activity.
Molecular docking simulations to understand substrate binding.
Comparative structural analysis of BSHs.

Main Results:

Detailed understanding of BSH structure and function is crucial for inhibitor design.
BSH substrate preferences are elucidated through structural and kinetic studies.
Molecular docking provides insights into the binding mechanisms of potential inhibitors.

Conclusions:

BSH inhibitors represent a promising, non-antibiotic alternative to AGPs in animal feed.
Rational design of BSH inhibitors can be based on existing structural and functional data.
Further research is needed to develop potent, safe, and cost-effective BSH inhibitors for the animal feed industry.