Basolateral amygdala inactivation eliminates fear-induced underestimation of time in a temporal bisection task

We examined interval timing - time perception in the seconds-to-minutes range - of the fear-inducing stimulus and the role of the amygdala in this phenomenon. Rats were initially trained to perform a temporal bisection task, in which their responses to levers A and B were reinforced following 2-s and 8-s tones, respectively. After acquisition, the rats were also presented with tones of intermediate durations and pressed one of the two levers to indicate whether the tone duration was closer to 2 or 8 s. Subsequently, the rats underwent differential fear conditioning, in which one frequency tone (conditioned stimulus; CS+) was paired with an electric foot shock, whereas another frequency tone (CS-) was presented alone. The rats were then infused with artificial cerebrospinal fluid (aCSF) or the GABA_A agonist muscimol into the bilateral basolateral amygdala (BLA) before performing the bisection task with CS+ and CS-. In rats infused with aCSF, the psychophysical function shifted rightward in CS+ relative to that in CS-. Moreover, the point of subjective equality of the CS+ was higher than that of CS-, suggesting that the duration of the fear -CS was perceived as shorter than that of the neutral CS. However, muscimol infusion into the BLA abolished this difference, suggesting that BLA inactivation suppresses the effect of the fear -CS. Our results demonstrate that normal BLA activity is essential for fear-induced underestimation of time.