Immobilized enzyme-based analytical tools in the -omics era: Recent advances

Area of Science:

• Analytical Chemistry
• Biochemistry
• Proteomics and Glycomics

Background:

• Protein analysis is rapidly advancing due to technological innovations like miniaturization and automation.
• The -omics era has driven interest in proteomics and glycomics, requiring efficient protein sample preparation.
• Enzyme-based analytical tools are evolving to overcome limitations of traditional in-solution enzymatic reactions.

Purpose of the Study:

• To review recent advancements in immobilized enzyme reactors (IMERs) for protein and glycoprotein analysis.
• To discuss the design and operational setups of IMERs.
• To highlight novel dual IMERs and tandem IMER platforms.

Main Methods:

• Review of recent literature on IMERs for protein analysis.
• Analysis of technological advances in enzyme immobilization and reactor design.
• Examination of applications in proteomics and glycomics.

Main Results:

• Development of efficient and miniaturized enzyme-based analytical tools, specifically IMERs.
• IMERs and IMER-based platforms show promise for automation and increased analysis throughput.
• Emergence of dual IMERs and tandem IMER platforms integrating multiple processing steps.

Conclusions:

• IMERs represent a significant advancement in automated protein and glycoprotein analysis.
• Ongoing research and development continue to enhance IMER efficiency and applicability in -omics fields.
• Dual and tandem IMER systems offer potential for streamlined and higher-throughput analytical workflows.