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Updated: Feb 6, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
Published on: September 5, 2019
Incorporation of STED technique into single-molecule spectroscopy to break the concentration limit of diffusing
Namdoo Kim1, Jiwoong Kwon, Youngbin Lim
1Department of Chemistry, Seoul National University, Seoul 08826, Korea. seongkim@snu.ac.kr.
Abstract:
By incorporating STED (stimulated emission depletion) nanoscopy into single-molecule spectroscopy, we demonstrate that the concentration limit imposed by optical diffraction can be overcome in diffusion-based single-molecule measurement. We showed that single-molecule detection is feasible at a concentration of 5 nM, which is 100-times higher than the limit of conventional single-molecule measurements.
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