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A tumor-targeting probe based on a mitophagy process for live imaging.

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A novel pH-sensitive fluorescent probe, glucosamine-modified cyanine dye CyT, was developed. This probe enables simultaneous imaging of mitochondria and autolysosomes and targeted tumor imaging in living cells.

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Area of Science:

  • Biomedical Engineering
  • Chemical Biology
  • Molecular Imaging

Background:

  • Mitochondria and autolysosomes play crucial roles in cellular processes.
  • Distinguishing these organelles based on their distinct pH environments is challenging.
  • Targeted imaging of tumors requires specific molecular probes.

Purpose of the Study:

  • To synthesize a novel near-infrared cyanine dye (CyT) modified with glucosamine.
  • To develop a pH-sensitive fluorescent probe for simultaneous imaging of mitochondria and autolysosomes.
  • To enable targeted tumor imaging using the glucosamine-modified probe.

Main Methods:

  • Synthesis of a glucosamine-modified near-infrared cyanine dye (CyT).
  • Evaluation of the probe's pH sensitivity.
  • Simultaneous imaging of mitochondria and autolysosomes in living cells.
  • Assessment of tumor-targeting capabilities.

Main Results:

  • Successfully synthesized a pH-sensitive near-infrared cyanine dye (CyT).
  • Demonstrated simultaneous visualization of mitochondria and autolysosomes based on pH differences.
  • Confirmed tumor-targeting potential due to glucosamine modification.

Conclusions:

  • The developed CyT probe is effective for simultaneous pH-dependent organelle imaging.
  • Glucosamine modification enhances the probe's utility for targeted tumor imaging.
  • This fluorescent probe offers a promising tool for advanced cellular and tumor imaging.