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Updated: Feb 6, 2026

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Published on: November 16, 2018
Nkd2 promotes the differentiation of dental follicle stem/progenitor cells into osteoblasts
Chanchan Chen1, Jianying Zhang2, Junqi Ling3
1Department of Stomatology, Shenzhen Children's Hospital, Shenzhen, Guangdong 518038, P.R. China.
Abstract:
Dental follicle stem/progenitor cells have the potential to undergo osteogenesis. naked cuticle homolog 2 (Nkd2) is a signal‑inducible feedback antagonist of the canonical Wnt signaling pathway. The purpose of the present study was to investigate the function of Nkd2 in the differentiation of dental follicle stem/progenitor cells (DFSCs) into osteoblasts. Immunohistochemistry, reverse transcription‑quantitative polymerase chain reaction and western blotting were employed to detect Nkd2 expression in rat DFSCs. In addition, rat DFSCs (rDFSCs) were transfected with small interfering RNAs to examine the effect of Nkd2 on the differentiation of these cells into osteoblasts. Furthermore, the function of Nkd2 in the Wnt/β‑catenin pathway in rDFSCs was investigated using β‑catenin/T‑cell factor luciferase activity assays and western blotting. It was revealed that the expression of Nkd2 was upregulated during the differentiation of rDFSCs into osteoblasts. Furthermore, osteoblast differentiation ability and Wnt/β‑catenin pathway activity were significantly decreased in Nkd2‑silenced rDFSCs compared with the si‑NC group (P<0.05 and P<0.001, respectively). The results suggest that Nkd2 promotes the differentiation of rDFSCs into osteoblasts through Wnt/β‑catenin signaling.
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