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Updated: Feb 6, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
An S-alkyl thiocarbamate-based biosensor for highly sensitive and selective detection of hypochlorous acid
1Key Laboratory of High Performance Polymer Materials and Technology of Ministry of Education, Department of Polymer Science & Engineering, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing, 210023, China. fengfd@nju.edu.cn.
Abstract:
We reported a near-infrared biosensor that features a dihydromethylene blue and an S-alkyl thiocarbamate linker to detect hypochlorous acid in a drastic fluorescence turn-on response. We achieved high sensitivity at the nanomolar level and high selectivity that resolves the interference issue with mercury ions and other transition metal ions. We demonstrated the response mechanism by analysing the released segments, and investigated the imaging capability to detect both exogenous and endogenous hypochlorous acid in living cells.
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