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A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
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Functional Biointerfaces Based on Mixed Zwitterionic Self-Assembled Monolayers for Biosensing Applications
Yu-Sin Wang, Shuehlin Yau1, Lai-Kwan Chau2
1Department of Chemistry , National Central University , Jhong-Li , Taoyuan 320 , Taiwan.
Langmuir : the ACS Journal of Surfaces and Colloids
|August 16, 2018
Summary
Zwitterionic carboxybetaine-thiols (CB-thiols) and sulfobetaine-thiols (SB-thiols) were synthesized to create functional self-assembled monolayers (SAMs) for biosensors. These modified surfaces enhance sensitivity and specificity in complex media, offering versatile antifouling properties for molecular recognition events.
Area of Science:
- Materials Science
- Biotechnology
- Surface Chemistry
Background:
- Biosensor performance, particularly sensitivity and specificity, is often limited in complex biological media.
- Surface modification strategies are crucial for improving biosensor functionality and reducing non-specific adsorption (fouling).
- Zwitterionic materials offer promising antifouling properties due to their charge distribution.
Purpose of the Study:
- To synthesize and characterize zwitterionic carboxybetaine-thiols (CB-thiols) and sulfobetaine-thiols (SB-thiols) for modifying gold substrates.
- To create functional self-assembled monolayers (SAMs) for enhanced immunoassay performance in surface plasmon resonance (SPR) biosensors.
- To evaluate the antifouling properties and biomolecule immobilization capabilities of these zwitterionic SAMs.
Main Methods:
- Synthesis of CB-thiols and SB-thiols.
- Surface modification of gold substrates to form SAMs.
- Characterization using X-ray photoelectron spectroscopy (XPS), contact angle goniometry, and cyclic voltammetry.
- Assessment of antifouling properties via protein and bacterial adsorption studies.
- Immunoassay development and performance evaluation on an SPR biosensor.
Main Results:
- SB-thiol SAMs exhibited superior hydrophilicity, fouling resistance, and packing density compared to CB-thiol SAMs.
- CB-thiols provided essential functional groups for biomolecule immobilization via amine coupling.
- Mixed SAMs of CB- and SB-thiols successfully integrated antifouling and functionalization properties.
- Zwitterionic mixed SAMs enabled efficient immobilization of biorecognition elements (BREs), leading to improved SPR biosensor sensitivity and specificity.
Conclusions:
- Zwitterionic mixed SAMs comprising CB- and SB-thiols offer excellent versatility, antifouling capabilities, and functionalizability for biosensing.
- This surface chemistry is highly effective for improving the performance of SPR biosensors in complex media.
- The developed surface modification strategy is broadly applicable for monitoring specific molecular recognition events.
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