Effects of diazepam on circadian phase advances and delays

Phase advances of hamster locomotor rhythms, which normally can be induced by light pulses in the late subjective night, were blocked in a dose-dependent manner by the benzodiazepine, diazepam. Light-induced phase delays were unaffected at doses that significantly blocked phase advances. Diazepam caused small phase delays of the free-running rhythm when given without a light pulse at either phase advance or phase delay time points. These results are discussed with regard to the possibility that different neurochemical mechanisms are required to process light-induced phase advances and delays and that GABA neurotransmission may be involved in the modulation of light input to the clock.