Related Experiment Video
Updated: Feb 6, 2026

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
Published on: May 16, 2012
Development of a TaqMan-based real-time PCR for detecting duck adenovirus 3
Chunhe Wan1, Cuiteng Chen1, Longfei Cheng1
1Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention/Fujian Animal Diseases Control Technology Development Center, Institute of Animal Husbandry and Veterinary Medicine of Fujian Academy of Agricultural Sciences, Fuzhou 350013, China.
Abstract:
Recently, a novel duck adenovirus (designated as duck adenovirus 3, DAdV-3) was discovered in Muscovy ducks, China. Here, we developed a TaqMan-based real-time PCR assay (qPCR) for the detection of DAdV-3 infection. After the optimization of the qPCR conditions, the lower limit of detection for DAdV-3 infection was 40.9 copies/μl. No cross-reactivity was observed with other duck-derived pathogens. Intra- and inter-assay variability was less than 2.30%. DAdV-3 was detected in embryos and newly hatched ducklings by qPCR assay, the findings provided evidence of possible vertical transmission of DAdV-3. The developed qPCR analysis showed high specificity, sensitivity, and reproducibility, thereby indicating that it can be used in future investigations on the pathogenesis and epidemiology of DAdV-3.
Related Concept Videos
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Piaget's Stage 3 of Cognitive Development
Conservation and Constancy of Quantity
A significant cognitive milestone in the...
PCR
ortho–para-Directing Activators: –CH3, –OH, –⁠NH2, –OCH3
Real Number System
Sustainable Development

