Related Experiment Video
Updated: Feb 6, 2026
10:19
Substernal Thyroid Biopsy Using Endobronchial Ultrasound-guided Transbronchial Needle Aspiration
Published on: November 10, 2014
26.3K
A biopsy-needle compatible varifocal multiphoton rigid probe for depth-resolved optical biopsy
Ang Li1, Gunnsteinn Hall1, Defu Chen1
1Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Journal of Biophotonics
|August 18, 2018
Summary
A new rigid probe enables three-dimensional two-photon optical biopsy within biopsy needles for internal organ imaging. This innovation significantly reduces motion artifacts during in vivo studies.
Area of Science:
- Biomedical Engineering
- Optical Imaging
- Medical Devices
Background:
- Minimally invasive imaging techniques are crucial for in vivo diagnostics.
- Two-photon microscopy offers high resolution but often requires bulky, inflexible setups.
- Existing endoscopic imaging lacks the resolution and depth penetration for detailed cellular analysis of internal organs.
Purpose of the Study:
- To develop a compact, biopsy-needle compatible probe for three-dimensional (3D) two-photon optical biopsy.
- To enable high-resolution, depth-resolved imaging of internal organs in vivo.
- To overcome limitations of current endoscopic imaging for internal organ diagnostics.
Main Methods:
- A rigid probe with a 1.75 mm outer diameter was designed to fit within a gauge-14 biopsy needle.
- A focus scanning mechanism and a 2D MEMS scanner were integrated for 3D imaging.
- High-numerical aperture micro-objective lens enabled high-resolution imaging with fast acquisition (up to 10 fps).
Main Results:
- The probe achieved a two-photon resolution of 0.833 × 6.11 μm (lateral × axial) with a 120 μm field of view.
- 3D imaging of mouse internal organs and subcutaneous tumors was successfully performed in vivo.
- The first demonstration of depth-resolved two-photon optical biopsy of a mouse kidney in vivo and in situ was achieved.
Conclusions:
- The biopsy-needle compatible rigid probe facilitates minimally invasive 3D two-photon optical biopsy of internal organs.
- The system's high resolution and fast imaging reduce motion artifacts, enabling detailed in vivo analysis.
- This technology represents a significant advancement for endoscopic imaging and internal organ diagnostics.
Related Concept Videos
00:49Rigid Body Equilibrium Problems - I
5.5K
A rigid body is said to be in static equilibrium when the net force and the net torque acting on the system is equal to zero. To solve for rigid body equilibrium problems, do the following steps.
5.5K
01:21Rigid Body Equilibrium Problems - II
8.0K
A rigid body is in static equilibrium when the net force and the net torque acting on the system are equal to zero.
Consider two children sitting on a seesaw, which has negligible mass. The first child has a mass (m1) of 26 kg and sits at point A, which is 1.6 meters (r1) from the pivot point B; the second child has a mass (m2) of 32 kg and sits at point C. How far from the pivot point B should the second child sit (r2) to balance the seesaw?
Consider two children sitting on a seesaw, which has negligible mass. The first child has a mass (m1) of 26 kg and sits at point A, which is 1.6 meters (r1) from the pivot point B; the second child has a mass (m2) of 32 kg and sits at point C. How far from the pivot point B should the second child sit (r2) to balance the seesaw?
8.0K
01:27Uniform Depth Channel Flow
590
Uniform depth channel flow keeps fluid depth consistent along channels such as irrigation canals. In natural channels, such as rivers, approximate uniform flow is often assumed. This condition occurs when the channel’s bottom slope matches the energy slope, balancing potential energy lost from gravity with head loss due to shear stress. This balance prevents depth changes along the channel length, resulting in a steady, uniform flow.Uniform flow in open channels with a constant cross-section...
590
01:11Angular Momentum: Rigid Body
15.9K
The total angular momentum of a rigid body can be calculated using the summation of the angular momentum of all the tiny particles rotating in the same plane. Considering all the tiny particles rotating in the x-y plane, the direction of angular momentum of all such particles and that of the rigid body would be perpendicular to the plane of the rotation along the z-axis.
This calculation can get complicated when tiny particles within the rigid body are not rotating in the same plane but have...
This calculation can get complicated when tiny particles within the rigid body are not rotating in the same plane but have...
15.9K
01:06Virtual Work for a System of Connected Rigid Bodies
761
Virtual work is a powerful method used to solve problems involving several connected rigid bodies. When the system is in equilibrium, virtual work is zero. This allows the calculation of the resulting forces when a system undergoes a virtual displacement. When attempting to analyze such a system, first, use a free-body diagram, where an independent coordinate represents the configuration of the links, and mark its deflected position resulting from the positive virtual displacement.
Next,...
Next,...
761
01:13Kinetic Energy for a Rigid Body
541
Imagine a solid object involved in a general planar movement, with its center of mass pinpointed at a spot labeled G. The object's kinetic energy relative to an arbitrary point A can be quantified for each of its particles - the ith particle in this case. This measurement is achieved through the employment of the relative velocity definition. The position vector, known as rA, extends from point A to the mass element i.
541