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Updated: Feb 6, 2026

Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci
Published on: March 4, 2021
Abstract:
This paper aims to develop an understanding of a peculiar hourglass-shaped caustic that can be observed if a partially filled wine glass is illuminated by parallel light. The light pattern is first described in terms of an idealized model of the liquid volume only: the sum of the parametric projection curves obtained by light refraction through liquid cones of varying wall inclinations in the framework of geometrical optics. Within this analysis, analogues to two specific refraction ice halos, the circumzenithal and the Parry arc, will prove to be helpful. The analysis is supplemented by ray tracing simulations that further elucidate the origin of the hourglass caustic when the glass is added to the refraction scenario.
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