Retinal tissue oxygen tension and consumption during light flicker stimulation in rat

Light flicker stimulation has been shown to increase inner retinal oxygen metabolism and supply. The purpose of the study was to test the hypothesis that sustained light flicker stimulation of various durations alters the depth profile metrics of oxygen partial pressure in the retinal tissue (tPO₂) but not the outer retinal oxygen consumption rate (QO₂). In 17 rats, tPO₂ depth profiles were derived by phosphorescence lifetime imaging after intravitreal injection of an oxyphor. tPO₂ profile metrics, including mean inner retinal tPO₂, maximum outer retinal tPO₂ and minimum outer retinal tPO₂ were determined. QO₂ was calculated using a one-dimensional oxygen diffusion model. Data were acquired at baseline (constant light illumination) and during light flicker stimulation at 10 Hz under the same mean illumination levels, and differences between values obtained during flicker and baseline were calculated. None of the tPO₂ profile metrics or QO₂ differences depended on the duration of light flicker stimulation (R² ≤ 0.03). No significant change in any of the tPO₂ profile metrics was detected with light flicker compared with constant light (P ≥ 0.08). Light flicker decreased QO₂ from 0.53 ± 0.29 to 0.38 ± 0.30 mL O₂/(min*100 gm), a reduction of 28% (P = 0.02). The retinal compensatory responses to the physiologic challenge of light flicker stimulation were effective in maintaining the levels of oxygen at or near baseline in the inner retina. Oxygen availability to the inner retina during light flicker may also have been enhanced by the decrease in QO₂.