Detection of Visually Imperceptible Blood Contamination in the Oral Surgical Clinic using Forensic Luminol Blood

Raniah Abdullah Al-Eid1, Sundar Ramalingam1, Chalini Sundar2

  • 1Department of Oral and Maxillofacial Surgery, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.

Abstract

Insights

Visually imperceptible blood contamination is common on surfaces and personal protective equipment (PPE) during oral surgery. Enhanced cleaning protocols are essential to prevent infection spread in dental clinics.

Area of Science:

  • Infection Control in Dentistry
  • Oral Surgery Safety
  • Biomedical Research

Background:

  • Oral surgical procedures can lead to the spread of infections via aerosolized and splattered blood.
  • Visually imperceptible blood contamination poses a significant risk in clinical settings.

Purpose of the Study:

  • To evaluate visually imperceptible blood contamination on clinical surfaces and personal protective equipment (PPE) during oral surgery.
  • To assess the effectiveness of luminol in detecting hidden blood traces.

Main Methods:

  • Oral surgical procedures were conducted in a disinfected clinic under local anesthesia.
  • Luminol was used to detect blood contamination on surfaces and PPE post-procedure.
  • Statistical analysis correlated procedure duration with contamination frequency.

Main Results:

  • 100% contamination of instrument trays, lights, dental chairs, and suction units.
  • Significant contamination (86.67%) on flooring below the surgical field.
  • Blood detected on most PPE and patient drapes; longer procedures (>40 min) increased glove contamination risk.

Conclusions:

  • Minor oral surgical procedures result in widespread, visually imperceptible blood contamination.
  • Routine cleaning and disinfection of surfaces and PPE are crucial before and after procedures.
  • Enhanced protective measures for clinicians and patients are necessary to mitigate infection risks.

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