Related Experiment Video
Updated: Feb 6, 2026

Murine Nasal Lavage Fluid Collection without Blood Contamination
Published on: July 11, 2025
Detection of Visually Imperceptible Blood Contamination in the Oral Surgical Clinic using Forensic Luminol Blood
Raniah Abdullah Al-Eid1, Sundar Ramalingam1, Chalini Sundar2
1Department of Oral and Maxillofacial Surgery, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Aim And Objectives:
Oral surgical procedures can cause spread of infections in the clinics through visually imperceptible, splattered, and aerosolized blood. The aim of this study was to evaluate visually imperceptible blood contamination of clinical surfaces and personal protective equipment (PPE) in an oral surgery clinic using luminol.
Materials And Methods:
Following ethical approval, oral surgical procedures were performed under local anesthesia in a disinfected clinic, and PPE was used by the oral surgeon, dental assistant, and patients. After the procedure, clinical surfaces and PPE were evaluated for traces of visually imperceptible blood contamination using luminol. Data regarding blood contamination and the duration of the procedure were collected. Nonparametric tests, with 95% significance level (Epi Info, Stat Calc 7, CDC, Atlanta, USA), were used to identify statistical interactions between the duration of the procedure and the frequency of blood contamination.
Results:
Blood contamination was detected in flooring below surgical field (86.67%), instrument tray, operating light, dental chair, and suction unit (100%). Except head caps and shoe covers, blood contamination was detected in all the PPE used by the clinical personnel, and the eyewear and chest drapes used by patients. An increase in the surgical time beyond 40 min significantly increased the risk of blood contamination in the handcuffs of the clinical personnel (P < 0.01).
Discussion And Conclusion:
Visually imperceptible blood contamination of the clinical surfaces and PPE is associated with minor oral surgical procedures. This mandates the cleaning and disinfection of all clinical surfaces before and after minor oral surgical procedures and PPE for clinicians and patients during every procedure.
Insights
Visually imperceptible blood contamination is common on surfaces and personal protective equipment (PPE) during oral surgery. Enhanced cleaning protocols are essential to prevent infection spread in dental clinics.
Area of Science:
- Infection Control in Dentistry
- Oral Surgery Safety
- Biomedical Research
Background:
- Oral surgical procedures can lead to the spread of infections via aerosolized and splattered blood.
- Visually imperceptible blood contamination poses a significant risk in clinical settings.
Purpose of the Study:
- To evaluate visually imperceptible blood contamination on clinical surfaces and personal protective equipment (PPE) during oral surgery.
- To assess the effectiveness of luminol in detecting hidden blood traces.
Main Methods:
- Oral surgical procedures were conducted in a disinfected clinic under local anesthesia.
- Luminol was used to detect blood contamination on surfaces and PPE post-procedure.
- Statistical analysis correlated procedure duration with contamination frequency.
Main Results:
- 100% contamination of instrument trays, lights, dental chairs, and suction units.
- Significant contamination (86.67%) on flooring below the surgical field.
- Blood detected on most PPE and patient drapes; longer procedures (>40 min) increased glove contamination risk.
Conclusions:
- Minor oral surgical procedures result in widespread, visually imperceptible blood contamination.
- Routine cleaning and disinfection of surfaces and PPE are crucial before and after procedures.
- Enhanced protective measures for clinicians and patients are necessary to mitigate infection risks.
Related Concept Videos
Oral Hypoglycemic Agents: Glinides
Oral Hypoglycemic Agents: Sulfonylureas
Oral Hypoglycemic Agents: Biguanides and Glitazones
Composition of Blood
Formed elements constitute the remaining 45% of the blood volume. These...
Blood Flow
Blood Types
ABO blood group
ABO antigens are glycoproteins encoded by genes present on...

