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Related Experiment Videos

Osteonectin mRNA: distribution in normal and transformed cells.

M F Young, M E Bolander, A A Day

    Nucleic Acids Research
    |June 11, 1986
    PubMed
    Summary

    Researchers isolated bovine osteonectin complementary DNA (cDNA) clones, confirming their identity through DNA sequencing. Osteonectin mRNA is present in bone and tendon but not in liver or brain tissues.

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    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Osteonectin is a bone-specific protein involved in bone mineralization and remodeling.
    • Understanding the gene expression of osteonectin is crucial for studying bone development and disease.

    Purpose of the Study:

    • To isolate and characterize complementary DNA (cDNA) clones encoding bovine osteonectin.
    • To investigate the tissue-specific expression of osteonectin mRNA in bovine tissues.
    • To examine the presence of osteonectin mRNA across different species and in viral-transformed cells.

    Main Methods:

    • Construction of a lambda gt11 expression library from bovine bone cell mRNA.
    • Isolation of overlapping cDNA clones encoding bovine osteonectin.
    • Hybrid select translation and DNA sequence analysis to confirm clone identity.

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  • Northern blot analysis to determine mRNA size and tissue distribution.
  • cDNA hybridization to detect osteonectin mRNA in various species and cell types.

    • Main Results:

    • Isolated overlapping cDNA clones encoding bovine osteonectin, with the longest clone (lambda On 17) measuring 2.0 kb.
    • Confirmed osteonectin gene expression in bone and tendon tissues, but not in liver or brain.
    • Detected osteonectin mRNA in human, rat, mouse, and chick cells, with significantly reduced levels in Rous sarcoma virus-transformed chick embryo fibroblasts.

    Conclusions:

    • The study successfully isolated and characterized bovine osteonectin cDNA, providing a tool for further molecular studies.
    • Osteonectin mRNA exhibits a distinct tissue distribution, primarily in bone and tendon, suggesting specific functional roles.
    • The presence of osteonectin mRNA across species and its altered expression in transformed cells indicate its conserved importance and potential involvement in cellular transformation.