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Updated: Feb 6, 2026
05:33
Isolation of Soil Microorganisms Using iChip Technology
Published on: January 10, 2025
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Pontibacter terrae sp. nov., isolated from soil.
Dong-Uk Kim1, Ju-Young Kim1, Jun Hwee Jang1
1Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Summary
A novel bacterial species, Pontibacter terrae, was isolated from soil. This Gram-negative, aerobic rod exhibits unique chemotaxonomic traits, distinguishing it as a new member of the Pontibacter genus.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- The genus Pontibacter comprises Gram-negative, aerobic bacteria.
- Bacterial taxonomy relies on a combination of genotypic and phenotypic characteristics for species delineation.
Purpose of the Study:
- To characterize a novel bacterial isolate, strain 17SD1-15T, from soil.
- To determine the taxonomic position of strain 17SD1-15T within the genus Pontibacter.
Main Methods:
- Isolation and cultivation of bacterial strain 17SD1-15T from soil.
- Gram staining, aerobic cultivation, and analysis of major fatty acids and polar lipids.
- Genomic DNA G+C content determination.
- 16S rRNA gene sequencing and phylogenetic analysis.
- DNA-DNA hybridization for relatedness studies.
Main Results:
- Strain 17SD1-15T consists of Gram-negative, aerobic rods.
- Major fatty acids include iso-C15:0 and summed feature 4. Polar lipids comprise phosphatidylethanolamine, one phospholipid, and five unidentified lipids.
- The G+C content of genomic DNA is 49.0 mol%.
- Phylogenetic analysis based on 16S rRNA gene sequences shows relatedness to other Pontibacter species, with highest similarity (98.3%) to Pontibacter saemangeumensis GCM0142T.
- DNA-DNA relatedness between strain 17SD1-15T and the most closely related Pontibacter type strain was 42.9±0.8%, indicating it represents a distinct species.
- Genotypic and phenotypic data, including chemotaxonomic traits, differentiate strain 17SD1-15T from known species.
Conclusions:
- Strain 17SD1-15T represents a novel species within the genus Pontibacter.
- The proposed name for this new species is Pontibacter terrae sp. nov.
- The type strain is 17SD1-15T (=KCTC 52915T=NBRC 113057T).
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