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Updated: Feb 6, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
Enhancing candicidin biosynthesis by medium optimization and pH stepwise control strategy with process metabolomics
Xiaoyun Liu1, Xiaojuan Sun1, Tong Wang1
1State Key Laboratory of Bioreactor Engineering, School of Biochemical Engineering, East China University of Science and Technology, Room 413, Building 18, Shanghai, 200237, People's Republic of China.
Abstract:
Candicidin is one of the most effective antimonilial agents. In order to enhance candicidin productivity, medium optimization and pH stepwise control strategy in process optimization were conducted by Streptomyces ZYJ-6. With the aid of Design Expert software and N/C/P-sources regulation, chemically defined medium fit for cell growth and candicidin biosynthesis was developed. Moreover, pH effects on cell growth and metabolism were investigated. The results indicated that the optimal pH for cell growth and candicidin biosynthesis were 6.8 and 7.8, respectively. The metabolomics analysis revealed that the pH stepwise control strategy (pH 6.8-7.8) combined the advantages of pH 6.8 and pH 7.8 and avoided precursor limitation in pH 6.8 and 7.8. Consequently, the pH stepwise control strategy played positive performance on cell growth and candicidin biosynthesis with the maximum titer of 5161 µg/mL. The titer of 5161 µg/mL was the highest level ever reported for candicidin production, which laid a solid foundation for industrial application. Additionally, pH stepwise control strategy was important reference for process optimization.
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