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[Multicomponent Content Difference Among Different Processing Degrees of Moutan Cortex Charcoal].

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    This study developed an HPLC method to analyze key compounds in Moutan Cortex charcoal. Processing significantly alters gallic acid, 5-HMF, catechin, and paeonol levels, impacting quality.

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    Area of Science:

    • Pharmacognosy
    • Analytical Chemistry
    • Traditional Chinese Medicine

    Background:

    • Moutan Cortex charcoal is a processed form of Moutan Cortex, used in Traditional Chinese Medicine.
    • Understanding the chemical changes during charcoal processing is crucial for quality control.

    Purpose of the Study:

    • To develop and validate a High-Performance Liquid Chromatography (HPLC) method for simultaneous determination of gallic acid, 5-hydroxymethylfurfural (5-HMF), catechin, and paeonol.
    • To investigate the quantitative variations of these compounds in Moutan Cortex charcoal at different processing degrees.

    Main Methods:

    • HPLC analysis using an Ultimate™ X B-C18 column.
    • Mobile phase: acetonitrile and water with 0.5% formic acid.
    • Detection at 245 nm, with a flow rate of 1.0 mL/min and column temperature of 30°C.

    Main Results:

    • Gallic acid and 5-HMF content initially increased with processing time, then decreased.
    • Catechin and paeonol content showed a decrease during the heating process.
    • Significant differences in compound content were observed across various processing degrees.

    Conclusions:

    • The established HPLC method effectively differentiates Moutan Cortex charcoal based on processing.
    • The findings provide a scientific foundation for establishing quality standards for Moutan Cortex charcoal.